Protein_Domain
Part:BBa_M50036:Design
Designed by: Amanda Urke Group: Stanford BIOE44 - S11 (2016-12-11)
6 Amino Acid Fusion Protein Linker
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimization software was used since we transformed our plasmid into E. coli. Also, the linker needed to be relatively short since we were already designing a long construct and needed to minimize the synthesis time given class constraints.
Source
Linker sequence was suggested and provided by our TA, Salil Bahte, and then optimized for use in E. coli.